Nitrite
Nitrite in water is a chemical compound containing the NO2– group and is produced from the oxidation of ammonia and the reduction of nitrate. It also plays an important role in biological and chemical processes.
Sources of Nitrite in Water
1- Decomposition of Organic Matter: It can result from the decomposition of organic matter in aquatic or soil environments.
۲- Sewage: The entry of industrial or domestic wastewater into water sources can increase the concentration of this compound.
۳- Nitrogenous Fertilizers: The use of chemical fertilizers in agriculture may lead to the leakage of nitrite into groundwater and surface water.
How to store the sample?
In order to prevent bacterial conversion of NO2– to NO3–, it should be analyzed as soon as possible. Do not use acid for sample preservation. For short-term storage for 1 to 2 days, freeze at -20°C or store at 4°C.
Sample measurement method
It is measured colorimetrically using a spectrophotometer. It is determined by the formation of a reddish-purple azo dye at a pH of about 2. The spectrophotometric range is 10 to 1000 μg/L. The colorimetric system follows the Beer-Lambert law with a 1 cm light path at 543 nm. Higher concentrations of NO2– can be determined by diluting a sample.
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